Tension-sensitive kinetochore phosphorylation and the chromosome distribution checkpoint in praying mantid spermatocytes

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Tension-sensitive kinetochore phosphorylation and the chromosome distribution checkpoint in praying mantid spermatocytes.

Improper chromosome attachment to the spindle can lead to daughter cells with missing or extra chromosomes. Such mishaps are avoided in many cells by a checkpoint that detects even a single improperly attached chromosome. What is detected? A misattached chromosome is not under tension from opposed mitotic forces, and in praying mantid spermatocytes, direct experiments show that the absence of t...

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Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint

Some cells have a quality control checkpoint that can detect a single misattached chromosome and delay the onset of anaphase, thus allowing time for error correction. The mechanical error in attachment must somehow be linked to the chemical regulation of cell cycle progression. The 3F3 antibody detects phosphorylated kinetochore proteins that might serve as the required link (Gorbsky, G. J., an...

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Microsatellite markers for the praying mantid Ciulfina rentzi (Liturgusidae).

Nine polymorphic microsatellite loci were characterized from an enrichment library of the Australian praying mantid Ciulfina rentzi, a group with a unique reproductive morphology and behaviour. The number of alleles per locus ranged from three to 16 and heterozygosity from 0.24 to 0.94. These markers are the first microsatellites developed for any praying mantid. They will be useful for paterni...

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The spindle assembly checkpoint detects defects in spindle structure or in the alignment of the chromosomes on the metaphase plate and delays the onset of anaphase until defects are corrected. Thus far, the evidence regarding the presence of a spindle checkpoint during meiosis in male Drosophila has been indirect and contradictory. On the one hand, chromosomes without pairing partners do not pr...

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Tension Management in the Kinetochore

The kinetochore is the protein machine built at the centromere that integrates mechanical force and chemical energy from dynamic microtubules into directed chromosome motion. The kinetochore also provides a powerful signaling function that is able to alter the properties of the spindle checkpoint and initiate a signal transduction cascade that leads to inhibition of the anaphase promoting compl...

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 1997

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.110.5.537